연구성과 홍보

Dig Liver Dis. 2025 Oct;57(10):1927-1937.

Title : Multiomics insights into functional constipation: Exploring microbiome, metabolome, and lipidome independent of transit time

Authors : Ji Eun Ryu1, Seung Yong Shin2, Ji-Seon Ahn3, Jung Min Moon2, Hyun Jin Joo2, Jeongkuk Seo2, Jung Seop Kim1, Ye-Been Lee1, Yoonho Jeong1, Jae-Yeon Cho1, SoHee Park1, Seung Ki Kwok4, Hea-Jong Chung5*, Chang Hwan Choi6*, Hyung-Kyoon Choi7*

Affiliations :

1College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.

2Chung-Ang University College of Medicine, Department of Internal Medicine, Seoul, Republic of Korea.

3Honam Regional Center, Korea Basic Science Institute, Gwangju, Republic of Korea.

4Rheumatology Division, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea.

5College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea; Honam Regional Center, Korea Basic Science Institute, Gwangju, Republic of Korea; Department of Bio-Analysis Science, University of Science & Technology, Daejeon, Republic of Korea.

6Chung-Ang University College of Medicine, Department of Internal Medicine, Seoul, Republic of Korea.

7College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.

DOI: 10.1016/j.dld.2025.07.011.

Abstract :

Background/aims: Functional constipation (FC) is a prevalent form of functional gastrointestinal disorder with an elusive etiology. Despite numerous investigations into the role of the gut microbiome in FC, no study has yet integrated microbiome, metabolome, and lipidome profiling to characterize FC.

Methods: In this study, fecal samples were collected from patients with FC both before and after laxative administration to minimize the impact of intestinal transit time and to identify the characteristics unique to patients with FC. These samples underwent comprehensive microbiome, metabolome, and lipidome analyses to discern the unique properties of FC.

Results: Beneficial microbiota such as Anerobutyricum hallii, Blautia luti, Blautia wexlerae, Collinsella aerofaciens, and Fusicatenibacter saccharivorans were predominantly found in healthy controls (HC), while harmful microbiota, Blautia faecis, was prevalent in patients with FC. Metabolome profiling showed that chenodeoxycholic acid, glucose, glycine, malic acid, phenylalanine, ribose, serine, and uracil decreased in patients with FC, suggesting a decreased energy metabolism and a reduced nutrient utilization for energy production compared to HCs. In terms of lipidome profiling, no significant differences in lipid levels were observed between pre- and post-laxative FC samples.

Conclusions: Our findings offer novel insights into FC-specific alterations in the gut microbiota and metabolome and potentially lead to the development of targeted therapeutic approaches for FC.